坝上长尾鸡MITF基因核心启动子鉴定与分析

doi:10.11843/j.issn.0366-6964.2018.06.003

Abstract

Abstract:

The research was designed to study the activity and the structure of MITF promoter and to provide clues for studying this gene's regulation mechanism in hair follicle. Dual-luciferase expression vectors were constructed and transfected to DF1 cells with lip2000. The dual-luciferase detection kit was used to measure the relative luciferase activity. The 6 expression vectors with different promoter regions of MITF gene and 4 mutant vectors of the core promoter region were constructed in Bashang Long-tail chicken. The core promoter region from -660 bp to +200 bp was identified in chicken MITF gene. The function of -579 bp, -505 bp, -274 bp, -220 bp, -203--202 bp, -98 bp, -46 bp, -14 bp, +1 bp and +28 bp sites in regulating promoter activity was critical. The similarities of transcription factor binding sites including HOX family (HOXA3, HOXD8, HOXD9, HOXD10 and HOX11), NF-1, DBP, TFⅡD and TFⅡB to MITF were changed predicted by software.

CLC Number:

S831.2

LIU Xiao-hui, PENG Yong-dong, ZHOU Rong-yan, ZHANG Chuan-sheng, LI Xiang-long. Identification and Analysis of the Core Promoter of MITF Gene in Bashang Long-tail Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1116-1123.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.06.003

https://www.xmsyxb.com/EN/Y2018/V49/I6/1116

References

[1] 赵兵令,王海东,陈天直,等.小鼠毛囊不同生长周期中MITF下游色素相关基因的定位表达及相关性分析[J].中国生物化学与分子生物学报,2017,33(2):198-206.
ZHAO B L,WANG H D,CHEN T Z,et al.Localization and correlation analysis of MITF downstream pigmentation-related genes at different growth cycle of mouse hair follicle[J].Chinese Journal of Biochemistry and Molecular Biology,2017,33(2):198-206.(in Chinese)
[2] VACHTENHEIM J,BOROVANSKY J."Transcription physiology" of pigment formation in melanocytes:central role of MITF[J].Exp Dermatol,2010,19(7):617-627.
[3] STEINGRIMSSON E,COPELAND N G,JENKINS N A.Melanocytes and the Microphthalmia transcription factor network[J].Annu Rev Genet,2004,38(38):365-411.
[4] 黎钊,王平,洪为松,等.正常人黑素细胞MITF对酪氨酸酶相关蛋白的转录调控研究[J].医学研究杂志,2013,42(3):58-62.
LI Z,WANG P,HONG W S,et al.Effect of MITF transcriptional control on family of tyrosinase-related proteins in normal human melanocytes[J].Journal of Medical Research,2013,42(3):58-62.(in Chinese)
[5] 杨玉静,张丹瑾,聂瑞强,等.绵羊MITF-M在黑素细胞中过表达后的功能分析[J].中国农业科学,2016,49(21):4214-4221.
YANG Y J,ZHANG D J,NIE R Q,et al.The function analysis of over-expression of oar MITF-M in melanocytes[J].Scientia Agricultura Sinica,2016,49(21):4214-4221.(in Chinese)
[6] 郑嫩珠,辛清武,朱志明,等.白绒乌鸡MITF基因的cDNA克隆、表达及其对黑色素沉积的影响[J].中国农业科学,2015,48(18):3711-3718.
ZHENG N Z,XIN Q W,ZHU Z M,et al.cDNA cloning and expression of MITF gene and its effect on melanin deposition in silky fowl[J].Scientia Agricultura Sinica,2015,48(18):3711-3718.(in Chinese)
[7] MARKAKIS M N,SOEDRING V E,DANTZER V,et al.Association of MITF gene with hearing and pigmentation phenotype in Hedlund white American mink (Neovison vison)[J].J Genet,2014,93(2):477-481.
[8] 牛晓艳,任克良,曹亮,等.獭兔MITF基因部分外显子多态性研究[J].山西农业科学,2013,41(11):1231-1234.
NIU X Y,REN K L,CAO L,et al.Research of polymorphisms of parts of exons in MITF gene in rex rabbit[J].Journal of Shanxi Agricultural Sciences,2013,41(11):1231-1234.(in Chinese)
[9] HARTMAN M L,CZYZ M.MITF in melanoma:mechanisms behind its expression and activity[J].Cell Mol Life Sci,2015,72(7):1249-1260.
[10] 张廷焕.猪MITF基因转录调控区域克隆及活性分析[D].雅安:四川农业大学,2014.
ZHANG T H.Cloning and activity analysis of pig microphthalmia-associtated transcription factor (MITF) gene promoter[D].Ya'an:Sichuan Agricultural University,2014.(in Chinese)
[11] KNUTSON B A,HAHN S.TFⅡB-related factors in RNA polymerase I transcription[J].Biochim Biophys Acta,2013,1829(3-4):265-273.
[12] LIU X,BUSHNELL D A,WANG D,et al.Structure of an RNA polymerase Ⅱ-TFⅡB complex and the transcription initiation mechanism[J].Science,2010,327(5962):206-209.
[13] ORPHANIDES G,LAGRANGE T,REINBERG D.The general transcription factors of RNA polymerase Ⅱ[J].Genes Dev,1996,10(21):2657-2683.
[14] ROEDER R G.The role of general initiation factors in transcription by RNA polymerase Ⅱ[J].Trends Biochem Sci,1996,21(9):327-335.
[15] 张爱贤,夏云飞.普通转录因子TFⅡB与肝细胞癌相关的研究进展[J].南通大学学报:医学版,2011,31(1):58-61.
ZHANG A X,XIA Y F.Advances in research on the association of general transcription factor Ⅱ B and hepatocellular carcinoma[J].Journal of Nantong University:Medical Sciences,2011,31(1):58-61.(in Chinese)
[16] KATSUMA S.Transcription of dbp from the coding region of the Bm17 gene is required for the efficient propagation of Bombyx mori nucleopolyhedrovirus[J].Virus Res,2016,223:57-63.
[17] 吕杨,陈蕾,谢院生,等.转录因子DBP对系膜增殖性肾炎免疫炎症反应的作用及其机制研究[C]//中国中西医结合学会肾脏疾病专业委员会2015年学术年会资料汇编.南宁:中国中西医结合学会肾脏疾病专业委员会,2015:846.
LV Y,CHEN L,XIE Y S,et al.The role of transcriptional factor DBP in immune inflammatory response to mesangial proliferative nephritis and its mechanism[C]//Information Compilation of the 2015 Academic Annual Meeting of the Professional Committee on Kidney Disease in Chinese and Western Medicine Society.Nanning:Chinese Academy of Integrated Traditional Chinese and Western Medicine Kidney Disease Specialized Committee,2015:846.(in Chinese)
[18] BABAJKO S,GROYER A.Interplay of the liver-enriched trans-acting factors,DBP and HNF1,in the transactivation of human IGFBP-1 promoter[J].Biochem Biophys Res Commun,1993,196(1):480-486.
[19] 张涛,张子平,贾锡伟,等.杂色鲍HSP90基因启动子的功能分析[J].水产学报,2017,41(4):490-497.
ZHANG T,ZHANG Z P,JIA X W,et al.Functional analysis of the promoter in Haliotis diversicolor HSP90 gene[J].Journal of Fisheries of China,2017,41(4):490-497.(in Chinese)
[20] 李丽莎,彭永东,郑晓宁,等.山羊PMEL基因启动子活性及转录调控元件分析[J].畜牧兽医学报,2017,48(5):826-835.
LI L S,PENG Y D,ZHENG X N,et al.Analysis of the promoter activity and transcriptional regulatory elements of goat PMEL gene[J].Acta Veterinaria et Zootechnica Sinica,2017,48(5):826-835.(in Chinese)
[21] MORGAN R,SIMPSON G,GRAY S,et al.HOX transcription factors are potential targets and markers in malignant mesothelioma[J].BMC Cancer,2016,16(1):85-96.
[22] 金淑清,浦予飞,裘莹.HOX基因的研究进展[J].癌症进展,2011,9(2):154-158.
JIN S Q,PU Y F,QIU Y.Hox genes mechanisms[J].Oncology Progress,2011,9(2):154-158.(in Chinese)
[23] 李慧,花保祯.Hox基因及其进化机制的研究进展[J].动物学杂志,2011,46(1):136-142.
LI H,HUA B Z.Hox genes and their evolutionary mechanisms[J].Chinese Journal of Zoology,2011,46(1):136-142.(in Chinese)

Identification and Analysis of the Core Promoter of MITF Gene in Bashang Long-tail Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	XU Xiaojing, DONG Ruiling, WEI Limin, ZHAO Shaomeng, ZHAO Guiping, ZHANG Minhong, FENG Jinghai. Study on Growth Model of Wenchang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4952-4961.
[3]	QI Lina, LU Xuelin, YANG Kaixuan, ZHOU Jin, LI Xianyu, LIU Ke, ZHANG Wengang, GU Xin, ZHANG Weijian. Analysis of Genetic Diversity and Genetic Structure of New Pudong Chicken Based on SNP Chips [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4962-4971.
[4]	WANG Yanxing, ZHANG Yushi, JI Haigang, LIU Yang, NIU Yufang, HAN Ruili, LIU Xiaojun, TIAN Yadong, KANG Xiangtao, LI Zhuanjian. Establishment and Analysis of the Chicken Skeletal Satellite Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4972-4981.
[5]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[6]	CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.
[7]	JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.
[8]	CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.
[9]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[13]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[14]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[15]	WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.